See wermelinglab.com/green-listed for more information.
To get you started you can try the following two demo alternatives:
Using the included GeCKOv2 Reference Library:
Using another Reference Library:
Note:The sample Reference Library (demo_source_list.txt) file contains only a few genes and cannot be used to designing a functional library as the gRNA sequences and scores are random.
Detailed Information
Doench JG*, Fusi N*, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J*, Root DE*. (Corresponding authors *) Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Feb;34(2):184-91. doi:10.1038/nbt.3437. Epub 2016 Jan 18. Link
Upload a Reference Library | info
Format: Tab Delimited Text File.
Enter the genes to query. Press Enter after each one.
Allow for partial matches
Row count: 0
Syntax: {Name}:{Sequence}
Example:
BsaI:ggtctc
BsmBI:cgtctc
This project was financially supported by the Swedish Research Council, the Swedish Foundation for Strategic Research, the Wenner-Gren Foundations, the Jeanssons Foundations, BILS (National Bioinformatics Infrastructure for Life Sciences), and the Karolinska Institutet.
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