See wermelinglab.com/green-listed for more information.
To get you started you can try the following two demo alternatives:
Using the included GeCKOv2 Reference Library:
Using another Reference Library:
Note:The sample Reference Library (demo_source_list.txt) file contains only a few genes and cannot be used to designing a functional library as the gRNA sequences and scores are random.
Doench JG*, Fusi N*, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J*, Root DE*.
(Corresponding authors *)
Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9.
Nat Biotechnol. 2016 Feb;34(2):184-91. doi:10.1038/nbt.3437. Epub 2016 Jan 18.
Upload a Reference Library | info
Format: Tab Delimited Text File.
Enter the genes to query. Press Enter after each one.
Allow for partial matches
This project was financially supported by the Swedish Research Council, the Swedish Foundation for Strategic Research, the Wenner-Gren Foundations, the Jeanssons Foundations, BILS (National Bioinformatics Infrastructure for Life Sciences), and the Karolinska Institutet.
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